As reviewed by Agarwal et al. However, these early meta-analyses were considered a near impossible task leading to vague and questionable conclusions. A major consideration for standardizing a protocol is to identify the SDF thresholds. This test is considered unreliable for the sensitive human clinic 9. Thus, AO likely penetrates the entire highly compact nuclear chromatin structure 9 , Agarwal et al.
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Sperm Chromatin Structure Assay (SCSA)
SCSA Diagnostics: SCSA Test
Diagnosis of male infertility has mainly been based on the World Health Organization WHO manual-based semen parameter's concentration, motility and morphology. It has, however, become apparent that none of these parameters are reliable markers for evaluation of the fertility potential of a couple. A search for better markers has led to an increased focus on sperm chromatin integrity testing in fertility work-up and assisted reproductive techniques. During the last couple of decades, numerous sperm DNA integrity tests have been developed. These are claimed to be characterized by a lower intraindividual variation, less intralaboratory and interlaboratory variation and thus less subjective than the conventional sperm analysis. However, not all the sperm chromatin integrity tests have yet been shown to be of clinical value. So far, the test that has been found to have the most stable clinical threshold values in relation to fertility is the sperm chromatin structure assay SCSA , a flow cytometric test that measures the susceptibility of sperm DNA to acid-induced DNA denaturation in situ.
Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility
Evenson, L. Jost, D. Marshall, M. Zinaman, E. Clegg, K.
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